Development and Metabolic Characterization of Horse Gram (Macrotyloma uniflorum Lam. (Verdc.)) Mutants for Powdery Mildew Resistance

Horse gram is one of the lesser-known beans widely grown in India. One hundred and twenty-three homozygous horse mutants were screened for powdery mildew (PM) disease resistance using grade 0 to 4. The grouped based on level 2 (resistant) susceptible (3 4). PM altered chlorophyll fluorescence (a/b ratio), maturity duration, yield attributing traits. loss ranged from 4.55% 72.66%. After affirming level, resistant mutant (RM) with minimum (scale:0) (SM) maximum (scale:4) used metabolomic analysis through GC-MS. infection induced expression 66 metabolites representing 32 functional classes. number unique classes RM SM was 13 11, respectively, while eight common. A fold change common indicated an enhanced accumulation amine, alcohol, ester RM. Along pathogen-induced defensive metabolites, produced silane fluorene, whose biological significance unknown. Though expressed defence-related bio-molecules, it failed better.